Discover How BAPtofect®-25 Is Providing Superior Results For Insect Researchers
BAPtofect®-25 is a revolutionary new transfection reagent offering significant advantages for insect transfection over existing products. Comprised of branched amphipathic peptide capsules (BAPC®), an entirely new class of nanocarrier, the kit overcomes many of the common transfection issues associated with lipid and polymer-based products.
BAPtofect®-25 is:
- Non-toxic to insects
- Verified safe for honeybees by EPA method toxicology study
- Capable of binding with proteins, dsRNA, oligonucleotides, plasmids and CRISPR-Cas9
- Able to provide oral delivery of lethal dsRNA
- Proven to transfect CRISPR products beyond the germline
- Water soluble
- Extremely stable in high temperatures
Transfecting with BAPtofect®-25 yields improved efficacy and accuracy in your experiments. Not only will you experience better distribution of your payload, but the lack of cytotoxicity results in virtually no cell death. Injection near ovaries produces heritable germline gene editing in subsequent generations. This makes it possible to edit genes across arthropods and bypasses the need for microinjection of eggs, which typically results in their death.
BAPtofect®-25 is also available with encapsulated florescent dyes to visually confirm cell and tissue transfection.
BAPC® Distribution
Left: Distribution in mosquito larvae soaked in a BAPC® solution
Right: BAPC® distribution in a psyllid digestive system following oral application
published research
Delivery of Lethal dsRNAs in Insect Diets by Branched Amphiphilic Peptide Capsules
Results
"BAPtofect®-25 systems have revolutionized our transfection process and allowed our undergraduate researchers to transfect dsRNA within the first week of being in our lab. Amazingly easy and accurate."
- Dr. James Balthazor
Assistant Professor of Chemistry
Fort Hays State University